NICHOLSON, GS; RODGERS, BD; KELLEY, KM: Peripheral Glucoregulation in the Longjaw Mudsucker: Insulin Regulation of Muscle Glucose Transport Teleost fishes have often been referred to as “glucose intolerant”; and at one point doubt was cast on whether they possessed an insulin-stimulated glucose transport system in peripheral tissues (e.g., in muscle) comparable to that of mammalian […]

MCCORMICK, S.D.; BJ�RNSSON, B.T.; MOECKEL, A.M.; LERNER, D.T.; O’DEA, M.F.: Endocrine pathways for the disruption of the parr-smolt transformation by estradiol and nonylphenol. Sex steroids are known to interfere with normal development of the parr-smolt transformation, and environmental estrogens such as nonylphenol have recently been implicated in reduced returns of Atlantic salmon in the wild. […]

FENTRESS, J. F.; BART, H. L., Jr.; CHEEK, A. O.: Endocrine effects of paper mill effluent in longear sunfish Endocrine disrupting compounds are exogenous agents that interfere with the normal functioning of the endocrine system. Paper mill effluent is believed to contain endocrine disrupting compounds that affect sex steroid and protein levels, as well as […]

MAURES, TJ; ROYER, T; DUAN, C: Structure, developmental expression, and physiological regulation of zebrafish insulin-like growth factor binding protein-1 In this study, we have cloned and characterized the full-length insulin-like growth factor binding protein-1 (IGFBP-1) cDNA from zebrafish. The full-length 1032 bp cDNA encodes a protein of 283 amino acids. The 263 residue mature zebrafish […]

SHIMIZU, M.*; SWANSON, P.; HARA, A.; DICKHOFF, W.W.: Purification of a serum insulin-like growth factor binding protein from chinook salmon, Oncorhynchus tshawytscha Circulating insulin-like growth factors (IGFs) are bound to multiple binding proteins. IGF-binding proteins (IGFBPs ) modulate biological activities of IGFs by controlling availability of IGFs to their receptors. In salmon, at least three […]

DURICA, D.S.; WU, X.; HOPKINS, P.M.: Protein-Protein and DNA Binding Properties of a Crustacean Ecdysteroid Receptor. To facilitate physical characterization of their binding properties, we have cloned into expression vectors the entire coding regions of the two components of the Uca pugilator ecdysteroid receptor homolog, UpEcR (Genbank AF034086) and UpRXR (GenBank AF32983). GST-pull down experiments […]

SCHMIDT, KE; DESAI, P; SAK, KM; BALOGH, LM; KELLEY, KM: Membrane Binding of an Insulin-like Growth Factor (IGF)-binding Protein (IGFBP) in a Reptilian Heart Cell: Impact on IGF-I Action A heart cell line (IgH2) derived from Iguana iguana secretes a 30-kDa IGFBP that also localizes to the cell membranes. Competition binding assays on soluble iguana […]

DING, J; DUAN, C: Looking at IGF binding protein-2 actions through the zebrafish eyes Insulin-like growth factors (IGFs) are evolutionarily conserved peptides that are essential for normal animal growth. Recent studies utilizing cell cultures suggest that the bioactivity of IGFs could be modulated by specific, high-affinity IGF-binding proteins (IGFBPs). The in vivo roles of these […]

BERG, LK; DESAI, P; AROPE, SA; KELLEY, KM: Insulin-like Growth Factor-binding Protein-2 (IGFBP-2) and Angiotensin II (AngII) in Glucose-activated Murine Kidney Mesangial Cells With high ambient glucose (e.g., in diabetes), glomerular mesangial cells show activated proliferation and an over-production/accumulation of extracellular matrix (ECM). These changes lead to impaired glomerular filtration and renal failure. Associated changes […]

KIRSCHNER, L. B: The Mechanism of Sodium Uptake in Crayfish. Recently, we reported that 3 proton pump inhibitors, known to block Na-H exchange in frog skin had no effect on proton movement and little on Na uptake in tapwater-adapted (TW)crayfish. In contrast, all 3 reduced Na-H exchange in salt-depleted (SD) crayfish. It appeared that the […]