Shark skin is covered with denticles that create micropatterns. Many shark skin inspired surfaces have non-overlapping micropatterns that have been shown to be antifouling. However, the overlapping and irregularly shaped denticles of shark skin, which control adhesion of bacteria, have not been replicated on bioinspired surfaces. In our experiments, we developed shark skin inspired surfaces with ridge-covered overlapping protrusions resulting in variable roughness. We hypothesized that migration and adhesion of Staphylococcus aureus and Staphylococcus epidermis would be reduced on these rough surfaces compared to a flat commercial bandage and flat control due to surface interactions such as Van der Waals forces, steric, and electrostatic interactions, which are known to influence bacterial settlement. Skin samples from bonnethead sharks (Sphyrna tiburo) were micro-CT scanned and had roughness between 11 and 53 denticles / mm2. We developed surfaces resulting in five different roughness factors between 1.972 and 16.026 μm using PDMSe elastomer and assessed for growth of S. aureus and S. epidermis. Surfaces with similar morphology and roughness to bonnethead shark skin were significantly more effective at blocking bacterial adhesion and migration compared to a commercial bandage and flat PDMSe control. These surfaces have potential to reduce healthcare associated infections in hospital settings.