P13-2 Sat Jan 2 Using laboratory culture of the nudibranch Berghia stephanieae to study reproductive development and feeding behavior Taraporevala, N*; Goodheart, J; Masterson, P; Johnston, H; Babonis, L; Lyons, D; University of California, San Diego; University of California, San Diego; University of California, San Diego; University of California, San Diego; Cornell University; University of California, San Diego nftarapo@ucsd.edu
Historically, nudibranch molluscs have been useful for studies of animal behavior and neurobiology, but their life history is hard to study as most species cannot be cultured through the entire life cycle in the lab. The aeolid nudibranch Berghia stephanieae, often used to keep ornamental corals free of the anemone Exaiptasia pallida, can be cultured through many generations in the lab in inland aquaria. Using a precise culturing method to raise individual egg masses of known age along with a detailed staging system, we will accomplish the following goals: 1. Determine precisely how early in development Berghia first mate, using their ability to grow in isolation. Preliminary studies have indicated that mating occurs before gamete maturity is obvious. Although they are reciprocally copulating hermaphrodites, this suggests that individuals may exchange and store sperm before they can produce eggs. 2. Determine the specific stages of the maturation of gametes prior to oviposition. Due to their translucence, we have observed eggs forming in an organized pattern, making the animal appear gravid. 3. Determine the factors that allow Berghia to consume different prey species. Berghia have previously been observed to be stenophagous, preying solely on Exaiptasia. However, we have documented some Berghia preying on the anemone Nematostella vectensis in certain contexts, showing that their diet may be broader than originally thought. Collectively, these factors allow us to improve the culture of Berghia by learning about its reproductive development and feeding behavior.