Many animals are under tremendous predation pressures and have evolved various anti-predatory strategies, such as aposmatic signaling and anti-predator behavior, to decrease predation. These strategies increase an animals’ fitness by allowing them to survive and reproduce. However, studies on anti-predatory behaviors have largely focused on organisms that are palatable. Studies on whether toxic species that possess aposematic coloration exhibit additional behavioral avoidance of predators, are largely lacking. Heliconius butterflies are toxic, unpalatable, aposematic, exhibit roosting behavior, and form Mullerian mimicry rings to avoid bird predation. However, specialized bird predators like Rufous-tailed Jacamar and Eastern Kingbird often feed on these butterflies, decreasing the efficiency of their unpalatability. Previous studies have identified auditory organs in Heliconius butterflies, but the functional significance of these organs are not known. We hypothesized that Heliconius butterflies may alter their behavior after hearing the calls of their avian predators. We used focal sampling to observe behaviors of Heliconius melpomene plessini in response to predatory bird calls and compared it with behavioral responses to control bird call (Toco toucan) and white noise. We found that H. m. plessini males and females changed their behavior in response to Jacamar calls only. Males flew longer while females fluttered more in response to Jacamar calls. Both these behaviors have been associated with anti-predatory behavioral strategies in other butterflies. These might be anti-predatory behaviors in Heliconius butterflies as well. These results will advance our knowledge in behavioral ecology of toxic and aposematic species.