Teeth are essential part of a sensory system for monitoring bite force and properties of the masticated food. The mandibular teeth are innervated by the Inferior Alveolar Nerve (IAN). Our study uses the XROMM workflow to track hemi-mandibular movements during mastication cycles by five opossums (Didelphis virginiana), and to test how IAN controls jaw kinematics. A helical axis method was used to calculate the axis of rotation of each hemimandible. In healthy individuals (with intact IAN), the axis of rotation is the same on both the left and right working sides (WS) and parallel and superior to the molar row during occlusion, and the molars were everting while translating lingually across the upper molars. The axis of rotation on the left and right balancing sides (BS) was medial to the anterior ramus and ran superio-anteriorly, moving the BS tooth row medially and the ramus laterally. However, after the left IAN was transected, the left and right hemimandibles exhibit different axes of rotation during WS occlusion. For the right hemimandible during right WS occlusion the axis of rotation is undefined (i.e., movement is too small to measure) or below the right hemimandible. The axis of rotation for the left hemimandible during WS occlusion is parallel and superior to the tooth row. These results suggest that bilateral sensory feedback through the IAN is important for control of hemimandibular rotation. Mastication is still possible without this feedback, but it is less efficient and differs from the intact condition. Grants: MRI 1338036, SR01DE023831-04S1, MRI1626552.