A fundamental question in ecology is how organisms are distributed in time and space. A critical determinant of this distribution is an animal’s locomotion because it affects multiple tasks that impact its fitness, including defense, dispersal, finding mates, foraging, and escaping from predators. Locomotion can be affected by the structural complexity of a habitat, obstacles, and escape strategies. This study examines the relationship between locomotor performance and habitat use in Six-Lined Racerunners (Aspidoscelis sexlineata) and will answer three questions: (1) Do Racerunners use their habitat randomly or non-randomly? (2) How does “available” habitat affect Racerunner locomotion? (3) Is the pattern observed in question #1 explained by question #2? We use habitat data collected via transects from Sullivan’s Island, South Carolina, and observations of Racerunners to quantify which substrates they run on in their natural habitat. To determine how available habitat affects locomotion, we conducted a lab experiment where Racerunners were placed on a racetrack covered in sand and/or debris and examined the acceleration, speed, and behavior that occurred during each trial. We then compared field habitat use to differential running performance and behavior in the laboratory.