The environment experienced by one generation can influence the phenotypes of future generations (transgenerational plasticity). Because parental cues can be conveyed to offspring at multiple points in time, ranging from at fertilization to post-emergence, offspring can potentially receive multiple cues from their parents via different mechanisms. We have relatively little information regarding how different mechanisms operate in isolation and in tandem, but it is possible, for example, that offspring phenotypes induced by epigenetic changes to gametes may be amplified by, mitigated by, or depend upon parental care. Here, we manipulated paternal experience with predation risk prior to fertilization in threespined stickleback (Gasterosteus aculeatus) and then examined the potential of paternal care to mitigate and/or amplify sperm-mediated paternal effects. Specifically, we compared offspring of predator exposed fathers who were reared with or without paternal care and offspring of control, unexposed fathers who were reared with and without paternal care. We found non-additive interactions between sperm-mediated and care-mediated paternal effects on offspring stress-induced cortisol: paternal predation exposure dampened stress responses in offspring, but only when offspring received paternal care. However, regardless of the presence or absence of paternal care, offspring were less active and daughters were larger when their fathers were exposed to predation risk, suggesting that paternal care neither amplifies nor compensates for these phenotypic effects induced by sperm. These results underscore the importance of considering how multiple mechanisms affect the outcome of transgenerational plasticity.