There is astounding functional diversity of pigments across insects. Fireflies (Coleoptera: Lampyridae), exhibit variation in body coloration and bioluminescence, yet the evolution of pigments remains unknown in this group. Using a comparative genomics approach, we investigated putative gene duplication events of pigment enzyme and transporter genes in the biosynthesis pathways of two pigment classes. To determine if duplicated genes are adaptive, we tested for selection in Photinus pyralis, a firefly species with aposematic coloration and yellow-shifted light emissions. As duplicates may gain divergent function, we assessed expression level of duplicates between sexes and developmental stages in the light producing organ of Photinus species. Future work will aim to address patterns of expression across various species and additional tissue types.