Maternal removal causes abnormal levels of fearfulness, antisocial behaviors, and learning, deficits in mammalian and avian offspring. In particular, zebra finches (Taeniopygia guttata) that experience maternal removal exhibit hyper-responsive hypothalamic-pituitary-adrenal (HPA) axis function. Around 80% of all bird species, including zebra finches, provide bi-parental care. Despite this, there have been few studies testing the effects of paternal removal on offspring. We set up groups of birds that experienced father removal at hatching, father removal at fledging, and a control group where both parents were present. Once mature, we obtained offspring baseline corticosterone through blood sampling. A week later, we subjected the same birds to a social isolation challenge and immediately took blood samples to obtain their stress-induced corticosterone levels. We predict that there will be no difference in baseline corticosterone levels between groups. We also predict that birds that experience paternal removal at hatching and at fledging will have higher stress-induced corticosterone levels in comparison to control birds. This study will help further our understanding in the significance of paternal care on the development of a normal stress response.