Colony collapse disorder (CCD), the leading cause of sudden death in Apis mellifera (Italian honey bees), is characterized by worker bees fortuitously leaving their colony in pursuit of nutrients and resources; however, these bees fail to return to the hives, ultimately leaving behind a queen, brood, and a small number of worker bees. While the primary cause of this phenomenon has not yet been discovered, agricultural pesticides, invasive Varroa mites, pathogens, and stress-related to habitat change are among the leading factors hypothesized to contribute to this phenomenon. The goal of this study was to observe how environmental and habitat changes due to colony migratory management, a common practice in which bee colonies are transported across the country in order to pollinate large- scale agricultural products, can affect the immunology of Italian honey bees over time since the period of colony establishment. Established colonies from the Dallas-Fort Worth area were transported and brought to St. Edward’s University. Started upon arrival of the bees, bees were collected and hemolymph was extracted biweekly to identify the impact on the bee’s immunological response. Common beekeeping techniques were also performed to simulate normal beekeeping operations as closely as possible. Samples were then subjected to several colorimetric assays to determine protein concentrations related to immune functioning. Preliminary data indicates that bees seemed to have an increase in overall protein concentrations, as well as prophenoloxidase (a protein commonly utilized by invertebrates in immune functioning) post-transport. Bee basal immune activity also seems to increase in response to environmental factors that may cause stress, such as thunderstorms. Samples are currently being subjected to assays to quantify the antioxidant peroxidase, with results forthcoming.