Reptiles use chemical signals from their environment to inform crucial behavioral processes, such as hunting prey or searching for mates. Previous studies found that male Burmese pythons (Python bivittatus), a major invasive predator in the Florida Everglades, can distinguish between scent trails laid by conspecifics and follow female trails. To further test this idea, we have collaborated with the National Park Service to assess the role of estrogens in activating female chemical signal production in pythons. The ultimate goal is to determine if pheromone development is a tractable management target. Male pythons were implanted with silastic implants containing either estradiol (E2; n=6) or left blank (SHAM; n=6). Wild, opportunistically caught male pythons (n=39) were then tested in a Y-maze to determine if they could follow either scent trail when left by an experimental animal in the maze. Many behaviors were observed during the trials (i.e., head shakes, pauses, turns, head raises). While the array of behaviors across the full trial (~12 hours) provides more insight on the patterns of wild male python behavior, those behaviors exhibited just until arm choice was made were prioritized for analysis. If wild males demonstrate more frequent behavior(s) toward E2 scent compared to SHAM scent, chemical feminization may have occurred which will be biochemically validated. The main outcome is the development an effective pheromone lure to enhance performance of trapping and removal efforts in South Florida.