Corticosterone (CORT) and melatonin (MEL) are hormones with complex immunomodulatory effects, including alterations in the temporal pattern of cytokine production. Cytokines are essential for advance and resolution of the inflammatory response. Pro-inflammatory cytokines (IL-1β and IL-6) provide leukocyte recruitment and activation for initial stages of the response, while anti-inflammatory cytokines (IL-10) inhibit the advance of inflammation at later stages, protecting against autoimmune damage. However, the dynamic of the inflammatory response is still underexplored in ectotherms. We investigated the progression of the inflammatory response in toads (Rhinella diptycha) injected with lipopolysaccharide (LPS; 2mg/kg) or saline. Toads were bled (1, 3, 6, and 18h post-injection) to measure CORT and MEL levels, and spleens collected to quantify cytokine mRNA expression. Our preliminary results for 1 and 6h post-injection show increased CORT and decreased MEL in LPS-treated toads compared to saline-treated 6h post-injection. We also found IL-1β and IL-6 upregulation in both 1h and 6h, but IL-10 was detected only 6h post-injection in LPS-treated toads. Alterations in CORT and MEL levels indicate activation of the hypothalamus-pituitary-interrenal axis and possible existence of the immune-pineal axis in amphibians, respectively. The cytokine expression pattern agrees with inflammatory progress since pro-inflammatory cytokines were expressed in both time-sets and the anti-inflammatory cytokine only in 6h. In further analysis, we expect higher IL-6 values and similar hormone levels in 3h, while for 18h, we predict lower IL1-β, IL-6, and MEL levels, followed by higher IL-10 and CORT levels in LPS-treated toads.