Meeting Abstract
50.4 Monday, Jan. 5 Skeletal muscle energetics following cold acclimation in a brown adipose tissue deficient mouse. MINEO, PM*; ROBERTS, ME; SCHAEFFER, PJ; Miami University; Miami University; Miami University mineopm@muohio.edu
We are interested in muscle plasticity in response to cold challenge. To remove the influence of brown adipose tissue (BAT) and non-shivering thermogenesis, we use the UCP-dta mouse, a transgenic line lacking BAT. When presented with an acute cold challenge, summit VO2 in UCP-dta mice were significantly lower than wild type controls. These results show that the lack of functional BAT in these mice greatly reduces their ability to produce heat during an acute cold challenge as shivering in skeletal muscle is not able to compensate for the absence of BAT. Further, we present data on the skeletal muscle mitochondrial respiration in these mice. Future experiments will investigate the effects of cold acclimation on the aerobic capacity of skeletal muscle following sustained shivering and how the increased activity due to shivering affects mitochondrial energetics.
