Immunology is the study of how organisms protect themselves against pathogens. The way the host’s body responds to pathogens is by using specialized cells and proteins to attack invaders, collectively called the immune system. Vertebrate immunology is an extensively researched area of biology; however, the innate immune response of invertebrates is still not well understood. For example, it is unclear if the cost of activating an immune response differs among species with conserved immune pathways, and therefore if the similar colorimetric immune assay protocols can be utilized across taxonomic groups of invertebrates to produce similar results. The two species studied in this project are Tigrosa helluo (a wolf spider) and Apis mellifera ligustica (the Italian honeybee). Because both species differ biologically, the same assay recipe may not be well-optimized for both organisms. This project aims to develop optimized assay recipes specific to each species for enzymes used with invertebrates when mounting an immune response including prophenoloxidase, and the antioxidants peroxidase and catalase. Once the assays have been optimized it is hypothesized that the spiders will have lower levels of enzyme activity proportional to body size when compared to bees. Because bees have a more social lifestyle, one can infer that their innate immune system is constantly being utilized due to community transmission of pathogens. In comparison, cursorial spiders primarily live a solitary lifestyle, and thus are predicted to have a lower frequency of immune challenges that would consequently increase immune enzyme activity.