Meeting Abstract

P3-11  Monday, Jan. 6  Quantification of BPA Retention in Planaria FLOOD, S*; DEPAOLA, N; MOODY, T; MASS, S; ST. JOHN, P; SUNY New Paltz; SUNY New Paltz; SUNY New Paltz; SUNY New Paltz; SUNY New Paltz floodb1@hawkmail.newpaltz.edu

Bisphenol-A (BPA) is a xenostrogenic environmental pollutant produced in large quantities by human industry, including plastic manufacturing and packaging for consumer goods, in health and beauty products and in the thermal printing industry. We have developed protocols using High Performance Liquid Chromatography (HPLC) and Gas Chromatography/Mass Spectrometry (GC/MS) to quantify BPA in freshwater planaria (G. tigrina) exposed to media containing known quantities of BPA. Continuing refinement of these methods has permitted us to measure BPA in pico molar quantities in planarian tissue. Prior work in our lab has demonstrated that exposure to Bisphenol compounds severely affects planarian behavior and regenerative capacity. These chromatography methods should now permit future work to correlate phenotypic abnormalities with specific quantities of BPA retained in tissue.