Meeting Abstract

P1.162  Friday, Jan. 4  Proteomic responses of Sebastes melanops to ocean acidification associated stress ZUZOW, M*; CHILTON, H; FANGUE, N; TODGHAM, A; TOMANEK, L; Cal Poly, San Luis Obispo mzuzow@calpoly.edu

Global climate change has implications for coastal marine ecosystems. Increasing CO₂ levels could have negative effects on physiological processes in numerous taxa including rockfishes (genus Sebastes). We chose the pelagic Black Rockfish (S. melanops) for our investigations for several reasons, including their economic and ecological importance in Pacific marine ecosystems, the availability of the species, its life history traits that make populations particularly sensitive to negative impacts, and the relative ease of collection and maintenance of its young life stages for laboratory experimentation. Among several goals of this experiment, we wanted to investigate the metabolic costs associated with developing under elevated CO₂ in juvenile fishes. Rockfish were exposed to 3 different CO₂ concentrations (400ppm, 1000ppm, and 2000ppm) over 7 d, 14 d, 21 d, 24 d, and 96d to monitor effects on development. We extracted protein from gill and liver tissues and separated their proteins with 2D gel electrophoresis. To identify protein expression patterns, we analyzed gels with Delta 2D (Decodon) and performed a 2-way permutation ANOVA to compare CO₂ concentrations and exposure times (p<0.02). Roughly a third of the proteins showed a time-dependent response to different CO₂ levels in both gill and liver tissues. Differing slightly, gill showed more of a time-independent response to increasing CO₂. The results suggest that there are broad similarities in the responses of the two tissue types. Proteins identified with tandem mass spectrometry in both tissues include proteins involved in the proteasome, oxidative stress proteins and proteins involved in energy metabolism.