Optimization of a new, biplanar X-ray video system for analyzing 3D kinematics and hydrodynamics of animals


Meeting Abstract

P2-210  Friday, Jan. 5 15:30 – 17:30  Optimization of a new, biplanar X-ray video system for analyzing 3D kinematics and hydrodynamics of animals VAN WASSENBERGH, S*; AERTS, P; Muséum National D’Histoire Naturelle, Paris; Univ. of Antwerp, Belgium svanwassenbergh@mnhn.fr http://samvanwassenbergh.weebly.com/

A biplanar, high-speed X-ray videography set-up for zoological research (named 3D2YMOX) was recently installed at the University of Antwerpen in Belgium. The set-up includes two Phillips Imagica image intensifiers, each coupled to a 4 megapixel, square-sensor Photron high-speed camera. We report tests of its performance: targeted volumes, spatial resolutions, and spherical marker tracking accuracies. A solution is presented to perform flat field corrections by using Photron’s pixel gain calibration function to equalize pixel intensities of an empty scene. In this way, the full dynamic ranges of the camera sensors can be used across the image field. Tests were performed with small particles (< 1.5 mm diameter) with a radio-opaque core that are neutrally buoyant in water. Such particles can be used to visualize flows of water in regions that have no optical access with standard cameras, e.g. on the inside of mouth cavities of aquatic animals.

the Society for
Integrative &
Comparative
Biology