Regeneration, the ability to replace lost body parts, is a widespread phenomenon in the animal kingdom that has been studied by biologists for a long time in a wide range of animals. This ability has been often connected to asexual reproduction, since the only difference between them appear to be the stimulus that triggers both processes. Hormones such as dopamine, melatonin and serotonin has been also related to control regeneration and asexual reproduction in many invertebrates. In particular, in annelids it has been proposed a hormonal control from the brain regulating both processes in a disjunctive way, but, up to now, any experiments with alive animals have been done. To shed light into this aim, we have investigated the hormonal control of these two trajectories in Pristina leidy, a freshwater oligochaete typically used as a model in developmental biology. The species exhibits huge regenerative abilities and routinely undergoes agametic reproduction by paratomic fission, i.e. the new animal is formed in the middle of a worm’s body before detachment. Based on previous studies, we have morphologically characterized both trajectories into different stages, stablishing 4 stages for anterior regeneration, 3 stages for posterior regeneration, and 5 stages for asexual reproduction. Finally, we have tested the influence of dopamine, melatonin and serotonin hormones on the regulation of regeneration and fission of the specie. We have also considered some environmental factors, such as light conditions, that seems to be decisive in hormone synthesis and release of other invertebrates. Our preliminary results show that both regeneration and asexual reproduction are slow down or even completely inhibited in the presence of hormone and in darkness.