Meeting Abstract

32.3  Monday, Jan. 5 08:30  Nitric oxide production and sequestration in the sinus gland of the green shore crab, Carcinus maneas, using a copper-based fluorescent ligand PITTS, N.L.*; MYKLES, D.L.; Colorado State University, Fort Collins; Colorado State University, Fort Collins pittsn@rams.colostate.edu

Molting in decapod crustaceans is regulated by molt-inhibiting hormone (MIH), a neuropeptide produced in the X-organ (XO)/sinus gland (SG) complex of the eyestalk ganglia (ESG). Pulsatile release of MIH from the SG suppresses ecdysteroidogenesis by the molting gland or Y-organ (YO). The hypothesis is that nitric oxide (NO), a neuromodulator that controls neurotransmitter release at presynaptic membranes, depresses the frequency and/or amount of MIH pulses to induce molting. NO synthase (NOS) mRNA was present in Carcinus maneas ESG and other tissues and NOS protein (~132 kDa) was present in the SG. A copper based ligand (CuFL), which reacts with NO to form a highly fluorescent product (NO-FL), was used to image NO in the ESG and SG and quantify the effects of NO scavenger (1 mM cPTIO), NOS inhibitor (1 mM L-NAME), and 1 mM sodium azide (NaN₃) on NO production in the SG. Preincubation with cPTIO prior to CuFL loading decreased NO-FL fluorescence ~30%; including L-NAME had no additional effect. Incubating SG with L-NAME during preincubation and loading decreased NO-FL fluorescence ~40%, indicating that over half of the NO release was NOS-independent. Azide, which reacts with NO-binding metal groups in proteins, reduced NO-FL fluorescence to near background levels without extensive cell death. Spectral shift analysis showed that azide displaced NO from a soluble protein in SG extract. These data suggest that the SG contains NO-binding protein(s) that sequester NO and releases it over a prolonged period. This NO release may modulate neuropeptide secretion from the axon termini in the SG. Supported by NSF (IOS-1257732).