Meeting Abstract
32.6 Jan. 5 Na+,K+-ATPase immunolocalization, activity and gene expression in Mugil auratus fry gills: after acclimated to artificial salinities KHODABANDEH, Saber*; SHAHRIARI MOGHADDAM, Mohsen; ABTAHI, Behroz; Univ. of Tarbiat Modarres, Tehran, IRAN surp78@gmail.com
The immunolocalization, activity, quantitative analysis of intensity and gene expression of the Na+,K+-ATPase in the gills of the golden grey mullet fry, Mugil auratus, investigated after acclimated in different salinity (<2‰, 12‰, 36‰ and 46‰). Na+,K+-ATPase enzyme detected in the gills of samples from all salinities. In <2‰, the fluorescent cells (ionocyts) were observed on the filaments, lamellae and in the epithelium of the gill chamber. The number and size of fluorescent cells decreased in the gills and in the epithelium of the gill chamber of samples from 12‰ salinity. Samples from the 36‰ and 46‰ were showed a high density of ionocyts on the gills and also on the epithelium of gill chamber. Na+,K+-ATPase intensity does not change significantly with salinity from 36‰ to 46‰ and it is significantly higher in the <2‰ salinity compared to 12‰. In compared to 12‰, the expression of Na+,K+-ATPase B1;1B1; gene increased 2, 2.5, and 3 fold in the gills of individuals transferred to <2, 36 and 46 ‰, respectively.
