Meeting Abstract
Adiponectin and its receptor (AdipoR) plays pivotal role in carbohydrate and fatty acid metabolism in vertebrates. We identified the full length cDNA encoding mammalian AdipoR homolog (Liv-AdipoR) from the shrimp, Litopenaeus vannamei. The full length Liv-AdipoR (1245 bp) encoded a protein with 415 amino acid residues. Liv-AdipR exhibited the conserved 7 transmembrane domains (7 TMs) and its predicted topology showed that Liv-APidoR belongs to the Progestin and adipoQ receptor (PAQR) family members. Based on the obtained sequences, only single AdipoR gene appear to exist in arthropods, while it has been evolved into two paralogs, AdipoR1 and AdipoR2 in vertebrates. Major production sites for Liv-AdipoR were the hemocyte, hepatopancreas, gonad and muscle and its expression was upregulated in those without feeding, which suggest that Liv-AdipoR may play a primitive role in carbohydrate and lipid metabolism as single gene. In order to estimate the function of Liv-AdipoR, dsRNA was designed and injected. After 72 hours of 50 pmol Liv-AdipoR dsRNA injection, its transcription levels decreased in thoracic muscle and deep abdominal muscle by 93% and 97%, respectively. Transcriptomic analysis showed that 804 contigs were upregulated and 212 contigs were down-regulated by the knockdown of Liv-AdipoR in deep abdominal muscle. Chronic effects of Liv-AdipoR dsRNA injection was the increased motalities suggesting function of Liv-AdipoR appears to be essential for survival of L. vanammei.
