Meeting Abstract
In mammals, adiponectin (also known as AdipoQ or Acrp30) is a protein that plays an important role in stimulating glucose utilization, fatty acid oxidation and improving insulin sensitivity. Adiponectin conveys its signals through the adiponectin receptors (adipoRs), which belong to G protein-coupled receptors (GPCRs). AdipoR is classified as PAQR (progestin and adipoQ receptors), which shows a unique inversed topology different from the typical GPCRs and has been paid attention for its medical implications. Since it plays key roles in glucose and fatty acid metabolism, AdipoR can be important targets for the various industrial purposes. In present study, we identified the full length cDNA encoding mammalian AdipoR homolog (Liv-AdipoR) from the shrimp, Litopenaeus vannamei, by the next generation sequencing and bioinformatics analysis. The full length Liv-AdipoR (1245 bp) encoded a protein with 415 amino acid residues. Liv-adipoR has high similarity to AdipoR from insect including Zootermopsis nevadensis (67%) and Apis dorsata (62%). Liv-AdipR exhibited the conserved 7 transmembrane domains (7 TMs) and its expression was ubiquitous. Change in expression level was also measured during molting and feeding cycles by qPCR. In order to estimate its function, sequence–specific Liv-AdipoR dsRNA (360 bp) was injected and its effects were studied. 10 pmol of dsRNA efficiently knocked down its expression by 74 % 3days after injection and Level of glucose and fatty acids in hemolymph was also measured. Long-term knockdown experiment was also performed injecting of 10pmol dsRNA weakly for 2 months and molting cycle and growth rate were measured.
