Cartilaginous shark vertebrae have a calcified double cone structure, a morphology that has been shown to impact mechanical properties. Adorning the double cone are mineral structures that vary among species. We aim to quantify mineral architecture of vertebrae from 18 species in three orders of shark (Lamniformes, Carcharhiniformes, and Squatiniformes). Previous literature has shown that posterior vertebrae are stiffer and tougher than anterior vertebrae. We hypothesize posterior vertebrae will have 1) greater quantity of lamellae and nodes (in applicable species), 2) smaller intermedialia angles, 3) larger centrum surface volume, and 4) higher mineral density than anterior vertebrae. Anterior and posterior vertebrae were dissected and scanned using a Bruker SkyScan 1173 μCT scanner. We measured the following morphological variables for each centrum: lamellae number, nodes along the lamellae, intermedialia angles, surface volume, and mineral density of the whole structure and a region of interest surrounding the remnant notochord. Preliminary data from Lamniform sharks show that posterior vertebrae mineral structure is composed of more lamellae and nodes when compared to anterior regions. For all species, mineral density was higher in the posterior region and we predict lower mineral values surrounding the remnant notochord. We hypothesize that the increased mineral architecture and higher mineral density found in the posterior regions contributes to stiffness and toughness of the cartilage, and the posterior vertebral column can contribute to more thrust production during swimming.