Meeting Abstract
63.3 Sunday, Jan. 5 14:00 Metabolism of cultured skeletal muscle of Coturnix quail selected for different rates of growth COOPER-MULLIN, C*; JIMENEZ, A.G.; ANTHONY, N. ; WORTMAN, M.; WILLIAMS, J.B. ; The Ohio State University; The Ohio State University; University of Arkansas; University of Cincinnati Cancer Institute; The Ohio State University cooper-mullin.1@buckeyemail.osu.edu
The connection between cellular physiology and whole-organism life history is a nascent field in Physiological Ecology. Growth rate is a fundamental parameter of an organism’s life history and varies greatly across bird species. Life histories in wild animals tend to fall on a “slow-fast” continuum with birds from the tropics in general falling on the “slow” end, where species tend to have low metabolic rates, prolonger nestling periods, longer lifespans, and lower investment in single reproductive events, whereas birds in temperate Ohio fall on the “fast” end with their higher rates of metabolism, short nestling periods, and shorter lifespans. To explore how growth rate and rate of metabolism of cells were connected to these whole-organism attributes, we used myoblast cells from Japanese quail (Coturnix coturnix japonica) that had been selected for fast or slow growth for over 60 generations. Cells from the fast-growth line had a significantly higher rates of basal oxygen consumption and glycolysis than the Coturnix Slow line. This led to the hypothesis that attributes of the mitochondria differ between the cell lines. Because mitochondria come from the mother, we tested the idea that mitochondria influence the rate of metabolism of these cells. We developed two hybrid lines, a fast male crossed with a slow female and a fast female line crossed with a slow male line. The data showed that the growth rate of the hybrid lines fell in between the growth rate of the fast line and the slow line. On the cellular level, the hybrid lines had intermediate rates of basal oxygen consumption and glycolysis, indicating that metabolic rates on the cellular level are not dictated by intrinsic properties of the mitochondria.
