A recent hypothesis has suggested that cuticular fluorescence may be plesiomorphic in Chelicerata (Arthropoda) despite its apparent absence from many arachnid lineages based on observations of whole-body (cuticular) fluorescence. Here, we explore the possibility of cuticular fluorescence in species of Acari (Ixodida), Amblypygi, Araneae, Opiliones, Schizomida, and Thelyphonida using resin-embedded, thin section histology and epifluorescence microscopy. We exposed histology sections of whole animals to different wavelengths of light (346 nm (UV), 488 nm, 546 nm) to determine if they fluoresce, and if the fluorescence is of a cuticular or non-cuticular (e.g., hemolymph) origin. We determined that members of most of the above taxa (though not all species) show evidence of cuticular fluorescence under all three wavelengths. Most UV and 488 nm fluorescence appears to be restricted to the exocuticle, though the specific lamina (pigmented or hyaline) that fluoresces will require higher resolution for clarity. Most species also show evidence of fluorescence in their endocuticle when exposed to 546 nm light. The strength of cuticular fluorescence in the different taxa was not quantified, but was concluded to be much lower relative to that of scorpions, which are well known to be strongly fluorescent under UV light. We think the low fluorescence signal of most non-scorpion arachnids explains why UV lamps cannot easily detect them in the dark.