Ranavirus infection is an emerging group of dsDNA viruses, which cause systemic infection of ectothermic vertebrates. The rising interest in Ranaviruses is due to sudden increases in range across the world and evidence of host-population declines. Ranavirus has been detected in China, associated with population declines of several species – species of conservation importance, the Giant Chinese salamander (Andrias davidianus , Critically Endangered), native species such as Rana dybowskii and Rana amurensis and several species of economic concern such as largemouth bass (Micropterus salmoides ) and tiger frog (Rana tigrina rugulosa ). Strategic monitoring in southern China is required to understand disease prevalence and determine species at risk. Sampling design focus on disturbed and undisturbed habitats across a latitudinal gradient together with opportunistic sampling of culture facilities and pet markets, known hotspots for the disease. Swabs taken from oral cavity, cloaca, or skin lesions and DNA were extracted from QAIGEN UCP Pathogen Mini kit. A set of primers and TaqMan (developed and partially validated to detect a panel of 33 different ranaviral isolates) were used. Our initial results indicate that some of the Chinese tiger frog (Hoplobatrachus cf. rugulosus ) from culture facility are infected with Ranavirus. This could be the first case of Ranavirus recorded in this region, which indicates that some of the cultured amphibian species could be responsible for spread of the disease. Understanding of disease ecology, anthropogenic influence, reservoir species is important in managing the disease to conserve species at risk.