Neurogenesis is the ability to generate new neurons from resident stem/progenitor populations. Although often understood as a routine or homeostatic process, various taxa of teleost fish, salamanders, and lacertid lizards are also capable of replacing neurons lost due to injury – so called reactive neurogenesis. Here, we investigated reactive neurogenesis in postnatal leopard geckos (Eublepharis macularius). To initiate reactive neurogenesis, a single dose of the antimetabolite 3-acetylpyridine (3-AP) was administered. Four days following 3-AP administration, there is widespread evidence of cell death and microglia activation within the medial cortex, the lizard homologue of the mammalian hippocampus. As evidenced by reduced expression of the neuronal marker NeuN, 3-AP appears to selectively target mature neurons. Within 30 days following 3AP administration, the medial cortex appears to be structurally restored with a pattern of NeuN expression that closely resembles the uninjured brain. Together, these data provide evidence that the capacity for reactive neurogenesis may be more taxonomically widespread among lizards than previously considered.