Vatalanib, a small molecule inhibitor of vascular endothelial growth factor receptor tyrosine kinases, is used in this experiment to inhibit blood vessel formation in the regenerating tails of axolotls. Ambystoma mexicanum is known for its remarkable capacity to regenerate severed appendages, making it an ideal organism in which to assess regenerative abilities in the absence of angiogenesis in the proliferative vs. maturation phases of tissue outgrowth. Here, we evaluate the relationship between vascularization and tissue outgrowth in terms of rate, outgrowth area and morphological quality of the regenerated tail. Comparison of the tail growth for each group shows no significant difference in the rate of tail regeneration (0.083mm/day vs 0.085mm/day for treatment vs. control, respectively) despite differences in vascularity. The data suggest little requirement for neovascularization in early tissue growth as both the control and treatment groups displayed comparable regenerative rates and outgrowth areas. The slight differences in tail morphology may, however, indicates the greater importance of angiogenesis in the later phases of tissue regrowth. This work provides a better understanding of the role of blood vessel formation on tissue renewal in a major regenerative species.