Meeting Abstract

74.3  Sunday, Jan. 6  Gene Expression and the diversification of Hawaiian crickets DANLEY, Patrick D*; SHAW, Kerry L; Baylor University; Cornell University patrick_danley@baylor.edu

Hawaiian crickets of the genus Laupala are a model evolutionary system in which the genetic basis of speciation may be examined. Within the past 5MY, 38 species of crickets in this genus have been identified. Closely related species differ in a limited number of phenotypes, the most notable of which is male song. The role of male song has been and continues to be examined with respect to mate recognition, sexual selection, and speciation. In addition to providing a rich evolutionary context, a number of genomic tools are available in this system. Linkage maps have been produced, QTL for male song have been identified, a gene index derived from the tissues responsible for the production and divergence of male calling is available, and a microarray based on this gene index has been produced. This study builds on these genomic resources by examining the developmental patterns of gene expression across three closely related species which differ in their song. Using a full factorial design, patterns of gene expression at 8607 unigenes were examined across three species and at three developmental stages during which the neural circuit responsible for male song is established. The results of this microarray study are discussed with respect to the divergence of species within Laupala.