Na⁺-dependent cation chloride cotransporters (CCCs) couple the inward movement of Cl^– to Na⁺ and/or K⁺. Aedes aegypti expresses three putative CCCs. AeNKCC1 is the ortholog of Drosophila Ncc69, a bumetanide-sensitive Na⁺-K⁺-2Cl^– cotransporter (NKCC), while aeCCC2 and aeCCC3 are orthologs of Drosophila Ncc83. In prior work, Xenopus oocytes expressing aeCCC2 exhibited increased Li⁺ uptake and Na⁺ currents, but equal Rb⁺ uptake, compared to water-injected controls (Kalsi et al., 2019). To further investigate the transport properties of NaCCC2s, we transiently transfected Sf9 cells with aeCCC2, Ncc83, and Ncc69 subcloned into pIB/V5-His vectors. In cells transfected with pHluorin-tagged Ncc83, plasma membrane expression was confirmed by fluorescent microscopy. Transport activity was assessed by exposing cells to saline with 20 mM [Li⁺ ] (Na⁺ tracer) and 5 mM [Rb⁺ ] (K⁺ tracer) and evaluating uptake via cation chromatography of cell lysates. Na⁺ to K⁺ ratios of cells transfected with aeCCC2 were 1.8 – 2.0 fold greater than controls and cells transfected with Ncc69. Following hypotonic pre-incubation, cells transfected with Ncc69 had 2-3 fold greater Rb⁺ uptake compared to controls. Additionally, cells transfected with aeCCC2 tended to have greater Li⁺ uptake, but equal Rb⁺ uptake, compared to vector-only controls. Our results support the hypothesis that, in contrast to Ncc69 and other NKCCs, aeCCC2 transports Na⁺ but not K⁺. (Funding: American Physiological Society Research Career Enhancement Award, NIH F33 GM131599, and Kenyon College.)