Hummingbirds are nectivorous birds that can uniquely fuel energetically demanding hovering flight with either recently ingested glucose or fructose. Hummingbird flight muscles posses both glucose and fructose transporters, thus allowing for direct fructose oxidation. However, the rates of fructose phosphorylation via hexokinase are not enough to sustain hovering flight. Thus, the utilization of sugars, particularly fructose, are unclear in hummingbirds. To elucidate this, we first determined whether fructose was present in the blood. Using LC-MS based metabolomics on the plasma of sucrose (glucose and fructose) fed hummingbirds we determined that fed hummingbirds had a blood fructose level of 5mM, compared to 0.2mM in fasted individuals. Glucose values between the two conditions stayed similar suggesting that fructose levels are relatively more dynamic. To explore the possible differential uses of fructose and glucose at the major organs, we compared ruby-throated hummingbirds fed glucose or fructose to fasted hummingbirds. We focused on the concentrations of central carbon metabolism metabolites in liver, heart and pectoralis muscle of these birds. We found that the hummingbird can use native fructose in the aforementioned tissues. However, there was surprisingly almost no difference between glucose fed and fructose fed birds, particularly at the pectoralis muscle. We found strong evidence that this was because a large portion of fructose is converted into glucose in the liver and shunted to the heart and pectoralis muscle for oxidation. Thus, the ruby-throated hummingbird not only possesses a higher capacity to use native fructose compared to mammals, but also seems to posses an extremely high capacity to rapidly convert fructose to glucose.