The axolotl, Ambystoma mexicanum, is an important model organism for the study of regeneration. After limb amputation a wound epidermis covers the surface of the stump and a regenerating bud of tissue or blastema forms, proliferates, and differentiates to restore all the limb elements that were lost. Although a blastema is only able to regenerate structures distal to its plane of amputation, when a wrist level blastema is grafted to a more proximal limb stump, it induces intercalary regeneration and a complete limb is restored. In contrast, creating the same proximodistal discontinuity, by grafting a freshly amputated hand or foot (autopod) to a more proximal limb stump, results in a truncated limb and does not induce intercalary regeneration. As a result, the mature autopod appears to lack the proper signals necessary to instruct the stump to fill in the gap, to dedifferentiate and intercalate the missing limb elements. These experiments create an opportunity to decipher the molecular signals responsible for intercalary regeneration and possibly regeneration itself. Through juxtaposition of beads charged with growth factors between a mature hand graft and more proximal limb stump it may be possible to determine the signals that initiate and drive intercalary regeneration in salamanders. Here we present a compilation of several studies in which we observed intercalary regeneration driven by fibroblast growth factor 10 as compared to control groups. However, graft displacement, amputation level and bead expulsion were all factors that appear to affect the regenerative patterns we observed. These results and future studies designed to optimize these elements will be discussed.