During the breeding season, a number of factors can affect female hormone levels. These include cyclical changes in ovarian hormones, nutrition, mate availability, and interactions among conspecifics. This study examines the effects of female social status on morphological traits and steroid levels in the brain and ovarian tissue of a hermaphroditic fish, the bluebanded goby, Lythrypnus dalli. In these fish, the male dominates the social hierarchy, which consists of a harem of females that compete for opportunities to reproduce. Here, we maintained social groups (n=34) consisting of one male and two size mismatched females for 4 weeks. The alpha female was subordinate to the male, but maintained a higher social status compared to the beta female. Alpha females had significantly higher standard length, body mass, and ovarian score compared to beta females. Overall, female status did not affect reproductive maturity, but there were differences based on ovarian stages. While levels of 11-ketotestosterone, testosterone, and cortisol in the brain were significantly higher in beta females compared to alpha females, levels of 17β-estradiol in the ovary was significantly higher in alpha females compared to beta females. Tissue hormone levels in females can be used to evaluate endocrine mechanisms that regulate reproductive physiology and behavior.