Meeting Abstract
P3.85 Thursday, Jan. 6 Fat chance: the effect of lipid supplementation on adapting to hypoxia in C2C12 skeletal muscle cells SCHLATER, AE*; CORLEY, A; DEMIRANDA, MA; KANATOUS, SB; Colorado State University; Pomona College; Colorado State University; Colorado State University amber.e.schlater@gmail.com
Endurance athletes are known for having high aerobic capacity in their skeletal muscle. Diving mammals are a unique type of endurance athlete that undergo apnea during exercise, yet still retain a high reliance on aerobic respiration in their primary swimming muscles. A hallmark adaptation that allows diving mammals to endure aerobic exercise in a seemingly hypoxic environment is the high abundance of myoglobin in the working skeletal muscle. Myoglobin regulation, however, is not well understood and further understanding of myoglobin regulation has important pharmacological implications for hypoxic conditions in human medicine. Previous research from our lab has employed cell culture to establish a correlation between lipid supplementation and myoglobin expression; specifically, increasing lipid supplementation in differentiation media of cell culture to the 5% level increased myoglobin expression in primary Weddell seal muscle cells under both normoxic and hypoxic conditions. The purpose of this study was to culture an immortal C2C12 cell line in 0.5% hypoxic conditions with 5% lipid supplementation in attempt to drive a diving phenotype in a terrestrial mammal. Cells from the lipid hypoxic line and a normoxic control line were homogenized and differential protein expression was analyzed using two-dimensional gel electrophoresis (2DE). In addition, myoglobin assays were used to quantify differences in myoglobin expression. Proteins were found to be differentially expressed between normoxic/glucose and hypoxic/lipid C2C12 cell lines. Myoglobin expression increased in hypoxic C2C12 cell lines with lipid supplementation alone, thus implying that lipids play a role in driving a diving mammal phenotype in a hypoxic terrestrial control.
