EMBRYONIC DEVELOPMENTAL EFFECTS OF MATERNAL INGESTION OF CYPERMETHRIN IN JAPANESE QUAIL Coturnix japonica


Meeting Abstract

P3-16  Monday, Jan. 6  EMBRYONIC DEVELOPMENTAL EFFECTS OF MATERNAL INGESTION OF CYPERMETHRIN IN JAPANESE QUAIL Coturnix japonica TELLEZ-GOMEZ, QM*; FLORES-SANTIN, JR; Universidad Autonoma del Estado de Mexico quetmaria@hotmail.com

Pyrethroid pesticides are chemical substances specifically used for the control of insects. Their effectiveness has contributed to its widespread use. However the toxic effects of this pesticides over other animal groups during agricultural practices has been put to question. Such is the case of cypermethrin, a pyrethroid with neurotoxic effects that induces slow growth, fertility issues, morphological and metabolic anomalies in amphibians. In Mexico cypermethrin is an authorized pesticide classified as highly harmful to humans. Birds from the Phasianidae family tend to be terrestrial foragers in crop fields, exposing them directly to the ingestion of the pesticide. The objective of this work is to assess the effects of maternal ingestion of cypermethrin in the development of the embryos. The use of Japanese quail provides a reliable model homologuous to wild populations of birds. A total of 5 females were used for each corresponding experimental group with 3 doses of cypermethrin (0.155g / kg, 0.315 g / kg, 0.6 g / kg) were administered through via feeding tube. A total of 40 eggs per group were collected evaluating mortality rate, weight, egg laying rate, fertility index and quality-length of eggs in females force fed cypermethrin. As well as dissecting at 5, 10, 15 days of embryonic development to obtain data on egg weight, height and diameter, venation, shell weight, yolk, chorioallantoic membrane and embryo, and morphometric data analyzed with Image J. Successful hatching specimens, as well as embryos on the 15th day of development of each experimental group, were sacrificed by inhalation to obtain data on weight, malformations and bone morphology by diaphanization, using the software Image J and statistical test. The data suggests an increased mortality as well delay in growth, and developmental anomalies.

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