Ampullary organs are electroreceptive sensory organs embedded in the lateral line system of many aquatic vertebrates and work by detecting electrical signals emitted into the water by other nearby organisms. These organs are used in migration, predator avoidance, and mate detection, but are most notably used to locate and acquire prey. While most research on ampullary organs has been conducted in teleost and elasmobranch fishes, aquatic salamanders also possess these specialized organs. Previous studies have given detailed histological descriptions of ampullary organs in salamanders, but very few have investigated the effects of this sensory information on an animal’s behavior. We exposed two-toed amphiuma (Amphiuma means) and three-toed (Amphiuma tridactylum) amphiuma salamanders to low-voltage, alternating and direct currents in water at different frequencies to test for behavioral indications of electroreception and potential feeding responses. Because amphiuma salamanders live in murky waters, are active at night, and spend long periods of time in burrows, sensory information other than sight may be important for movements and especially feeding. Our amphiumas showed behavioral responses, including likely feeding responses, to the electrical signals. These results will help determine the sensitivity and behavioral importance of ampullary organs in amphiuma salamanders, and highlight the value of testing for electroreception in other aquatic salamanders.