Meeting Abstract
P3.9 Tuesday, Jan. 6 Effects of Two Forms of EDTA, on Cadmium Accumulations in Gill of the Eastern Oyster, Crassostrea virginica YATES, M.*; CARROLL, M.A.; CATAPANE, E.J.; Medgar Evers College mona40t@yahoo.com
Cadmium (Cd) is a trace metal with no known biological functions. In humans it adversely affects a number of organs, including kidney, liver and lung by inducing apoptotosis or carcinogenesis. Routes of exposure include cigarette smoke and ingestion of cd contaminated food. Cd pollution is widespread in the aquatic environment and marine animals, especially bivalves, accumulate cd. The gill of the oyster Crassostrea virginica is a good tissue to study cd accumulations. Previously, we showed p-aminosalicylic acid was not effective in reducing cd accumulations. Sodium EDTA (EDTAna) was moderately effective. In clinical setting, calcium EDTA is the preferred form for metal chelation therapy. We sought to determine if there were a difference in efficacy of the 2 forms of EDTA in reducing cd accumulations. Gills were incubated for 10 hours with 500 µM cd then removed from the cd and incubated with EDTA for 3 days. Cd levels were measured using electrothermal vaporization with deuterium lamp background correction in a Perkin Elmer AA800 Atomic Absorption spectrophotometer with a THGA graphite furnace. Low concentrations of EDTAna (up to 1 mM) did not reduce cd accumulations; however, the higher concentrations (2 mM) was moderately effective in reducing the accumulations by about 40%. The EDTAca was significantly more effective in reducing cd accumulations, especially at low concentrations. A 2 mM concentration of EDTAca reduced accumulation by 60%. Even lower concentrations (500 µM and 1mM) produced significant reductions in cd accumulations, 25% and 40%, respectively. Cd accumulations cause serious toxic effects in animals and it can be valuable to find effective agents that can remove cd from tissues and blood. This work was supported in part by grants 0516041071 of NYSDOE, 0622197 of the DUE Program of NSF and 0420359 of the MRI Program of NSF.
