Meeting Abstract
P3.109 Jan. 6 Effects of donor age on cellular resistance to oxidative stress in short-lived versus long-lived rodents KRISTAN, Deborah M.; MANIBUSAN, Pierre T.*; GRIFFING, Paulina; California State University, San Marcos; California State University, San Marcos; California State University, San Marcos manib001@csusm.edu
Recent work by others has shown differences in cellular resistance to multiple forms of stress in control versus dwarf individuals of long-lived laboratory mouse (Mus musculus) strains using primary skin fibroblast cells. Moreover, donor age (neo-natal versus young adult) affected the ability of cells to resist oxidative and non-oxidative stress. Thus far, evidence that links whole animal lifespan with cellular resistance to oxidative stress in rodents has been primarily tested with genetically manipulated strains of M. musculus. Relatively little is known about cellular resistance to oxidative stress in naturally long-lived species of rodents. We compared resistance of kidney fibroblast cells to oxidative stress for wild-derived M. musculus (approximately 3-4 year lifespan) and deer mice and white-footed mice (Peromyscus maniculatus and P. leucopus, respectively; approximately 7-8 year lifespan) and found that cells from Peromyscus sp. were more resistant to paraquat and hydrogen peroxide exposure than cells from M. musculus. Ongoing experiments are comparing effects of donor age on ability of kidney fibroblast cells from an outbred strain of M. musculus and from cactus mice (P. eremicus) to resist oxidative challenge.
