Meeting Abstract

P2-56  Sunday, Jan. 5  Effects of Bisphenol-S and Estrogen on p53 Expression in Ovarian Tissue of Zebrafish (Danio rerio) FAIRBANKS, J*; DEMARAIS, A; University of Puget Sound jfairbanks@pugetsound.edu

Bisphenol A (BPA) is one the most widely used plasticizing compounds. As an endocrine disruptor, BPA could affect ovarian function and embryonic development. Bisphenol S (BPS) has been used in many plastics and resins in lieu of BPA. However, the effects of BPS are not widely known. Apoptosis is a type of genetically programmed cell death that is important in ovarian homeostasis. One of the genes that regulates apoptosis, p53, has been shown to respond to estrogen in maintaining ovarian homeostasis. We have investigated how exposure to estrogen and to BPS affects p53 expression in ovarian tissue in vitro. Zebrafish ovarian tissue was exposed to 0.01, 0.1, and 1.0 µM of BPS or estradiol (E2) and gene expression was quantified using qPCR. Results confirmed non-monotonic dose responses to both BPS and E2, with a trend of increased p53 expression at the higher concentration of BPS, while the higher concentration of E2 resulted in a trend of decreased p53 expression. These data suggest that BPS has the potential to affect ovarian homeostasis.