21-4 Sat Jan 2 Effects of bisphenol-A on the morphology and survival of larvae of the sand dollar Dendraster excentricus (Echinodermata, Echinoidea) Darin, EA; California State University Long Beach and Cabrillo Marine Aquarium, San Pedro, CA emily.darin@student.csulb.edu
Bisphenol-A (BPA) is an ingredient in the majority of plastics produced today. When plastics enter aquatic environments, BPA can leach out and affect the development and physiology of marine organisms. Comparisons of temperate and tropical species suggest that temperature has an impact on the sensitivity of marine organisms to BPA, but this hypothesis requires further testing, ideally using a single species that can be reared in a wide range of temperatures. The sand dollar Dendraster excentricus, which inhabits shallow waters from Baja California to Alaska, is one such species. In this preliminary study, I aimed to understand the effects of relatively low BPA concentrations on the larvae of D. excentricus. I exposed embryos and larvae of D. excentricus to four treatment solutions (no additives, vehicle control, 50 µg/L BPA, and 500 µg/L BPA) for seven days. The two BPA treatment concentrations are higher than have usually been detected in coastal marine waters, but lower than most other studies of BPA effects on echinoderm development. After exposure, I measured larval midline body length and postoral arm length, survivorship, and the frequency of normal development. Midline body length, postoral arm length, survivorship, and the frequency of normal development all decreased in the presence of BPA, in a dose-dependent manner. Relatively low concentrations of BPA can thus have strong effects on the development of sand dollar larvae. Larvae of this species, which can develop over a temperature range of at least ~12-22 °C, are thus a useful model for testing how sensitivity to Bisphenol-A (and possibly other pollutants) varies with temperature.