The ecomorphological paradigm states that a deterministic relationship exists between structure and function with regard to whole-organism performance capacities such as locomotion. Consequently, increasing functional demand should elicit changes in the underlying physiological and morphological pathways supporting that function. Previous research in green anole lizards showed that exercise training alters a variety of physiological and morphological characteristics of trained individuals, yet the specific molecular pathways that are upregulated to effect these changes are poorly understood. The insulin/insulin-like signaling (IIS) network, a highly conserved environmental sensing network that mediates growth and metabolism, is a likely regulator of muscle development and metabolism in response to increased anaerobic activity. Two of the primary hormones of this network are insulin-like growth factor (IGF) 1 and IGF2. IGF1 is an important catalyst for cellular growth, but IGF2 has been significantly understudied in adulthood. In this experiment, we sprint trained green anole females (Anolis carolinensis) for six weeks, thereby forcing them to increase allocation of energy resources to muscle growth. We tested the hypothesis that IGF1 and IGF2 expression are both upregulated in sprint trained animals because IGFs are important regulators of cellular reproduction and ultimately skeletal muscle growth. Specifically, we predicted that IGF1 and IGF2 hepatic gene expression would be upregulated in sprint trained lizards compared to untrained control animals. These results contribute to our understanding of the IIS network in general, and shed new light on the role of IGF2 in particular in regulating physiological plasticity in reptiles.