Eggshell maculation patterns have been studied extensively, leading to multiple adaptive explanations for the diversity in avian maculation pattern and egg size. This variation has often been attributed to signaling properties of eggshell patterns or habitat ecology (e.g., camouflage); however, our understanding of this variation is still somewhat limited. Our aim was to better understand the sources of intra- and inter-clutch variation in barn swallow (Hirundo rustica erythrogaster) egg size and maculation. We focused on two potential sources of variation within and between clutches: lay order as a source of intra-clutch variation in egg size and eggshell maculation, and breeding site, where we studied swallows breeding at organic, no-spray farms and near conventional agriculture. We photographed eggs (n=666) at multiple breeding colonies in two geographic locations (Georgia, Ohio). A subset of eggs (n=67) were labeled as they were laid to account for lay order. We predicted that the last-laid egg would look the most distinct from others in the clutch and that females breeding at no-spray farms would lay larger eggs. NaturePatternMatch was used to analyze the recognizability of patterns and calculate pattern similarity among eggs, and SpotEgg gave information including egg size, shape, number of spots, and spot distribution. Eggs typically resembled other eggs in the same nest more than eggs laid by other females. Additionally, the last laid egg in a clutch was consistently different in maculation compared to the earlier laid eggs. Breeding site influenced egg size such that females laid larger eggs at no-spray, organic farms.