Previous studies in a variety of vertebrates predict animals facing different arrays of stressors can exhibit an acute increase in circulating plasma glucocorticoid levels and consequent modulation of the immune response. However, studies investigating how the presence of parasites (external and internal) can affect the way toads deal with acute stressors is scarcely explored. In this study, we investigated if the presence of ectoparasites (ticks) might impact the stress biomarkers and the immune response in the Florida invasive cane toads (Rhinella marina). We measured the corticosterone (CORT) plasma levels and the neutrophil: lymphocyte ratio (NLR), two common stress biomarkers, and the bacterial killing ability (BKA), a common immune protein component, at baseline (field) and after a short-term stressor (24h restraint). Toads with parasites had higher baseline CORT levels, but the restraint effect was the same for all toads: increased CORT, NLR, and BKA. We also found a negative correlation between body index (BI) and CORT only in parasitized toads, indicating that higher levels of baseline CORT may be associated with a higher energy demand due to the ticks, which results in BI reduction. Nonetheless, ticks’ presence does not affect how toads respond to an additional stressor, nor the assembly of the immune response. Further studies, including different immune protocols and investigating the presence of endoparasites, might help to understand these relationships better.