Freshwater turtles, a long-lived group with multiple reproductive opportunities throughout their lives, must allocate finite energy and resources to reproduction, self-maintenance, growth, and basal processes. Energy allocation dynamics are expected to shift respective to clutch development, gravidity, and post-oviposition in females as these processes demand different profiles of resources and have varied energetic requirements. Further, clutch development investment may vary throughout the reproductive season in multiclutching individuals. We captured native red-eared sliders (Trachemys scripta), a multiclutching population, in the Delta Region of Arkansas and collected blood samples, recorded morphometrics, and conducted reproductive assessments via ultrasound between May and October 2019 and in June 2020. We performed bacterial killing assays and radioimmunoassays to assess innate immune function and hormone concentrations, respectively. Ovarian status was qualitatively scored based on presence of follicles, atretic follicles, and eggs. By determining how these abundant reptiles allocate their energy during obligatory processes, such as reproduction, we can better address questions within the fields of conservation and comparative ecophysiology.