Humans are capable of drastically altering their environment within a relatively short time frame, turning vast natural landscapes into bustling cities. Few organisms can adapt quickly enough to survive these changes; those who do often subsidize their diet with anthropogenic food since an animal’s natural food source may not be readily available. A species with populations living in both urban and rural environments may, therefore, experience musculoskeletal changes in response to different selective pressures stemming from food diversity, availability, and acquisition difficulty. Here, we compare skull dimensions and bite force of coyotes (Canis latrans) from an urban population (Greater Los Angeles) with those from a rural population (Fresno County). Upper jaw length and width, mastication muscle masses, lower jaw lever length, and skull length measurements were recorded for each specimen; and upper jaw length/width ratio and bite force at the carnassial molar and lower canine were calculated. Current findings indicate urban coyotes have greater variation in upper jaw shape, with statistically nonsignificant trends towards wider snouts, longer skulls and a stronger molar bite force in urban coyotes. While more data is needed, trends suggest urban environments favor the development or evolution of powerful bites to allow for feeding on domestic pets.