In a time when corals are facing massive mortality events, it is crucial to gain an understanding of the underlying symbioses between corals and their algal symbionts. The sea anemone Exaiptasia diaphana, commonly known as Aiptasia, has been widely used as a model system for understanding the cnidarian-dinoflagellate symbiosis because it can be maintained easily in a lab, it engages in a symbiosis and can be colonized with different algal species, and it can be maintained in an aposymbiotic state. In addition, Aiptasia undergoes a rapid form of asexual reproduction called pedal laceration, during which anemones leave behind a piece of the symbiotic pedal disk that will develop into a juvenile polyp. Laceration presents itself as a novel way to study the process of symbiosis establishment during host development. During juvenile polyp development, symbionts invade the developing tissue ultimately resulting in a fully symbiotic juvenile. The purpose of these experiments was to test expression of nutrient transporters as markers for symbiosis establishment during host development. We focused on the glucose transporters GLUT8, HMIT, and SMIT and lipids as markers for energetics by using fluorescence and bright field microscopy. We compared localization and abundance of glucose transporters and lipids in symbiotic vs aposymbiotic lacerates and tracked expression through time in symbiotic lacerates as they developed into juvenile polyps. Glucose transporters were detected with polyclonal antibodies against transporter proteins, and Oil Red O and BODIPY stains were used to visualize lipids in host tissues. Findings from these experiments will be crucial in furthering our understanding of animal development and symbiosis establishment in cnidarians.