Disturbance of microbial communities can have notable effects on biological systems due to the important ecological roles of microbes. For instance, symbiotic host-associated microbial communities impact host physiology and can be disturbed by many factors, including antibiotic exposure. Advancements in sequencing technologies have allowed ecological questions to be addressed more thoroughly in microbial systems. Previous research has shown that different microbial communities may respond differently to disturbance. This study addressed the impact of antibiotic perturbation on the bacterial communities in the honey bee gut microbiome. Honey bees are a good candidate for microbiome studies because they harbor gut communities with relatively low taxonomic diversity. We treated honey bees with low doses of tetracycline to identify how gut communities change following antibiotic exposure. Prior studies have demonstrated impacts of antibiotics in this system, but we used fecal sampling to account for variation in individual bees before and after exposure. Fecal samples were collected from individually-marked caged bees before, and five days after antibiotic treatment. We assessed changes in gut bacteria community structure using 16S rRNA gene amplicon sequencing. Our preliminary analysis suggests differences in the gut bacterial taxa present in the bees after disturbance with antibiotics.