Amphibious fishes, which spend any natural part of their life history out of water, are a subject of interest due to the adaptations which allow them to survive both on land and in water. In particular, the order Cyprinodontiformes contains many amphibious species. These species are closely studied because while they have many adaptations for living on land, their body plans are similar to that of a solely aquatic bony fish. This begs the question: do amphibious Cyprinodontiformes have subtle anatomical adaptations which allow them to better locomote and survive on land? Amphibious Cyprinodontiformes move on land primarily by performing a “tail-flip”, in which the anterior of the body curves up and over the posterior, and then the tail pushes off the ground to launch the fish into the air. Given this reliance on the spine and tail, the vertebral column and caudal region of amphibious and non-amphibious Cyprinodontiformes fishes were examined. In particular, the hypural plate, epurals, and parahypurals of the tail, and the neural and hemal spines throughout the vertebral column were compared. Previous studies have shown variation in jumping performance within an amphibious species that correlated with hypural and epural differences, and those patterns are expected to be upheld in this dataset. Geometric morphometrics was also employed to quantify body shape differences between species. By determining which anatomical changes allow for amphibious behavior, we will better be able to predict the present behavior of potentially amphibious fishes, and speculate at the behavior of extinct fish species.