Despite a recent surge in vaping related lung illness and an overall lack of research regarding the health implications associated with their use, electronic cigarettes and vaping devices have continued to remain popular among teens and young adults since their introduction to the United States over a decade ago. The popularity of these products among those of childbearing age necessitates research on the potential impact of maternal vaping on embryonic function during development. Here, we examined the effects of nicotine-free, cinnamon, or chocolate, flavored vapor on cardiovascular function during early development using the zebrafish model. Flavored electronic cigarette vapor was produced from a second-generation vaping device and was infused into dechlorinated water at high, medium, and low concentrations. Vapor infused water was distributed among flasks to which zebrafish embryos were added within 4 hours post fertilization. Videos of the heart and blood vessels were recorded at 24 hours post exposure and cardiovascular parameters were measured to assess the effects of cinnamon, or chocolate, flavored vapor on cardiovascular function. At high exposure concentrations, cinnamon flavored vapor significantly inhibited cardiovascular function while chocolate flavored vapor did not, thus indicating that cardiovascular function in the developing embryo may be affected in a flavor dependent manner, even in the absence of nicotine. The results of this study provide much needed data on the potential impact of flavored, nicotine-free, electronic cigarette vapor on cardiovascular function during early development which may occur as a result of maternal vaping during pregnancy.