Meeting Abstract

P2-182  Monday, Jan. 5 15:30  Attachment in an unstable environment: Quantification of the algal attachment protein vitronectin. FIELD, L.M.*; FAGERBERG, W.R.; BOETTGER, S.A.; West Chester University, Pennsylvania; University of New Hampshire; West Chester University, Pennsylvania lf776150@wcupa.edu

Algae form a diverse group of organisms that vary in morphology and physiology across the different representatives within the chlorophyta, rhodophyta, and phaeophyta. However, an extraction assay that will yield consistently high amounts of protein from these different organisms has yet to be determined. The current research sought to establish a high yield protein extraction assay and to determine the presence/absence of the multifunctional glycoprotein vitronectin in different algal species and different areas of the algal thallus. Five protein extraction assays were tested and the extraction method that yielded the highest amount of protein was used for extraction of protein from different thallus components.